Direct observations of routine patient test performance, including, as applicable, patient identification and preparation; and specimen coll ti h dli i d t tillection, handling, processing and testing 2. Attn: Specimen Processing. This phase begins in clinicians' offices with the initiation of the testing requisition and includes the steps for Guidelines for the Rejection of Specimens CLP 011-001 Revised March, 2007 1. • Specimens in which there has been a si gnificant time delay between specimen coll ection and specimen receipt (more than 24 hours), for which a transport tube/medium was not used and/or specimen results would be compromised. Specimen Processing Detection of bacterial pathogens from clinical specimens ... Specimens • Processing of suboptimal or poor quality specimens is a burden on both financial and personnel resources • Results generated from processing inappropriate specimens may not be reliable • Each laboratory must develop its own specimen rejection criteria and make these criteria readily Specimen Collection, Transport, and Processing ... Specimen Processing and Concentration of Chlamydia ... Storage/Transport: Transport to the Microbiology Laboratory immediately at room Empowered by bioMérieux, you can be sure it meets your unique needs. good standard of practice to which all clinical and public health microbiology # Microbiology is used as a generic term to include the two GMC-recognised specialties of Medical Microbiology (which includes Bacteriology, Mycology and Parasitology) and Medical Virology. PDF Lesson-13 Sample Collection & transport Specimen collection and processing is an important aspect of clinical microbiology laboratory. (PDF) Chapter 1 Specimen Collection, Processing, Culture ... PURPOSE OF EXAMINATION Diagnosis of urinary tract infection accomplished by semi-quantitative culture of urine and antimicrobial susceptibility testing of potential pathogens present in significant numbers. 5. improper specimen collection, specimen processing, or specimen transport. Experience in directing supervision, the Laboratory Assistant performs duties involving the processing of specimens, maintenance of . Heather Berger, Health Sciences . Microbiology Requisition . SPECIMEN MANAGEMENT IN DIAGNOSTIC MICROBIOLOGY Dr.T.V.Rao MD DR.T.V.RAO MD 1 2. Of similar importance, clinical microbiology specimen processing technologists must process according to the written procedure, as vigorous processing can kill certain molds such as Zygomycetes. About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features Press Copyright Contact us Creators . Specimens!! Specimen management in Microbiology 1. Introduction of a Chlamydia trachomatis L2 434 preparation containing 12 elementary bodies (EBs) into first-void urine (FVU) from 225 nonpregnant women and 190 pregnant women before specimen processing by the assays produced false-negative rates of 0.48% (2 of 415 specimens) and . When rare organisms such as Brucella, Campylobacter or Bartonella are suspected: An ID physician shall be consulted. 1,2: • samples (where possible) are taken before antimicrobial therapy is started • the need to process specimens within appropriate time scale for organism viability and clinical need • the safety aspects of specimen processing 2. specimens follow standard laboratory practices, including Standard Precautions, when handling potential COVID-19 patient specimens •Wear a lab coat in the lab, & remove before exiting. The Microbiology laboratory shall be consulted to advise which type of specimen is most likely to support the suspected organism. This chapter offers guidelines for specimen collection and transport, specimen handling, specimen pretreatment and processing in the laboratory, medium selection, and incubation of cultures. Manual of Clinical Microbiology, 11th Edition 4 28.2 Specimen Collection and Processing Precautions must be taken when collecting specimens from abscesses that may be caused by anaerobes (e.g., Bacteroides fragilis). From a list of commonly used microbiological media, identify the media that are used in the isolation and cultivation of mycobacteriae. Figure 28.1 A B 5 Common specimens include blood, pus, urine, sputum, throat, stool, and cerebrospinal fluid. Inhibitors in clinical specimens can be detected by adding the target of nucleic acid amplification to the sample. Biopsy 2.3 Microbiology Specimen processing . Quiz 6 :Specimen Collection and Processing. specimen can be processed, it is generally preferable to freeze the specimen at - 70°C. All specimens and/or cultures must be accompanied by a Campbell Microbiology Laboratory Requisition with patient demographics and insurance information, physician and location information, specimen source and laboratory testing requested. Specimen receipt and accessioning. › Facilitates automation and elutes samples in seconds. Cerebrospinal fluid (CSF) should be processed in a microbiology laboratory within 1 hour after collection or inoculated into Trans-Isolate (T-I) medium for transport to the laboratory if processing within 1 hour is not feasible. TML/MSH Department of Microbiology Policy #MI Policy & Procedure Manual \SPEC Page 1 of 15 Section: Microbiology Lab Specimen Management Manual Subject Title: Specimen Processing Chart Prepared by: QA Committee Original Date: May 10, 2001 However evidence shows most of the laboratory errors occur during the pre-analytical stage. Special Instructions: Specimen site and date/time of collection are required for processing. Department of Laboratory Medicine and Pathology 1959 NE Pacific Street, Room NW120 Seattle, WA 98195-7110 Tel: 206.598.6131 Fax: 206.598.6189 M-F 8am-5pm. This preanalytical phase of testing is critical… 302 General Principles of Specimen Collection and Transport . Microbiology Specimen Collection Guide Cellular Pathology (Histology & Non-Gynae Cytology Handbooks) 1.3.4 Guidance on Specimen and Container Type & Amount of Sample to Collect Please use the links given in section 1.3.3 above. Transport media: Some transport media's are available for microbiology specimen e.g. The questionnaire assessed selected characteristics of stool-testing practices in calendar year 2009, the type of testing for routine stool specimen workup, use of transport media for stool samples, specimen-processing time, and specifi c laboratory testing practices B The laboratory should . Prepare smear for Gram stain by placing a drop of specimen on a slide and spreading it to make a thin preparation. The processing technologists must transfer the submitted tissue onto the agar plates for the optimal recovery of filamentous fungal pathogens. Specimen Collection . 1. A Guide To Specimen Management In Clinical Microbiology by J. Michael Miller / 2017 / English / PDF. infective agents is one of the most important functions of the microbiology laboratory. Clean catch MSU- Mid stream urine is used because the first . Specimen collection • Proper specimen collection is one of the most important factors in diagnosing infection • Follow instructions for collecting and transporting specimens for microbiology tests • The best specimens are tissue, aspirate, pus, body fluid • Swabs are low yield and prone to contamination E-swab Acute endocarditis. This regional laboratory is one of the largest reference laboratories for the Ministry of Health. bacteria. The ideal specimen is an aspirate from a previously undrained abscess, or a tissue biopsy. Clinical Microbiology Laboratory only for the collection of specimens for processing by DLO. . PL-Microbiology Specimen Collection Guidelines (2020.07.22) Microbiology Specimen Collection Guidelines b. The fellow will be assessed for demonstrated proficiency in obtaining library and Internet resources. American Society for Microbiology, Washington DC Miller, J Michael (1999) A Guide To Specimen Management in Clinical Microbiology, American Society for Microbiology, Washington DC Microbiology Media, Tests and Specimen Processing. WASP ®: Walk Away Specimen Processor Automated microbiology specimen processing instrument. MICROBIOLOGY - FULL SERVICE EXAMPLE - POOR TEST SYSTEM DELINEATION Competency elements: 1. Classify specimens most likely to be received for culture of mycobacteriae. •Wear gloves when handling specimens. Automation holds the potential to improve workflow in the clinical microbiology laboratory by freeing skilled staff to performed more technical tasks, and by absorbing expanded operations without the need for additional staff. • Specimens in which the quantity is not sufficient for proper processing. After Hours: Samples accepted at Specimen Processing Services. performed on serum, plasma, or whole blood. 4.1.2 The specimen label does not match the label on the attached request form or mislabeled. Obtain 3 blood cultures with 3 separate venipunctures over 1 to 2 hours. Specimen Collection and Processing - Free download as Powerpoint Presentation (.ppt / .pptx), PDF File (.pdf), Text File (.txt) or view presentation slides online. Upon receipt, the CPA staff will check requisitions for completeness. Identification of the. Specimen Processing: Day 1: Aspirate, pus and swab: Mix the specimen thoroughly. d. The clinical microbiology laboratory is responsible for providing guidance on how to select, collect, label, and transport specimens for processing in the laboratory. One of our clients is looking for a Medical Laboratory Assistant- Microbiology specimen processing exp. Consider it dirty. be mixed with an equal volume of 50% or 70% ethyl alcohol and placed in refrigerator in microbiology laboratory to be delivered the following working day. Answer. A list of available in-house and commonly referred tests can be found in Section 8 (General Microbiology) and Section 9 (Virology) of this document. From blood cultures, bacteria, body fluids, swabs, and more, the lab handles a lot of specimens. In the laboratory, errors can occur at any stage of sample processing; pre-analytical, analytical, and post analytical stages. Introduction Reliable specimen preparation is crucial to diagnostic accuracy. WASP ® DT allows the reassignment of valuable, often scarce, laboratory staff without compromising the quality or reproducibility of microbiology cultures. PROCESSING AND INTERPRETATION OF CEREBROSPINAL FLUID SPECIMENS. For example, use 70% alcohol followed by iodine solution (1 to 2% tincture of The receipt and processing of specimens is . c. No 24-hour sputum collections-fresh, preferably early-morning specimens are collected for AFB processing and fungus culture. specimen processing By converting microbiology samples to a liquid format in standardized tubes, laboratories enjoy maximum utility from WASP DT automated specimen processor From planting and streaking to Gram slide prep and enrichment broth inoculation, WASP DT is the only instrument • Identify the specimen source and/or specific site correctly so that proper culture media will be selected during processing the laboratory. 5. Stuart's media, Cary-Blair media. Simulated clinical specimens are also used to teach the microbiology of infection. Urine All voided specimens should be collected as a mid-stream clean catch. Specimen Collection and Transportation of Microbiology Specimens Date & Time Viewed: 12/14/2010 at 11:54:42 AM Page 3 of 25 01/25/2010 Version 2 i. Request PDF | Specimen Collection, Transport, and Processing: Bacteriology | One of the key principles of good specimen collection is to avoid introduction of colonizing bacteria surrounding the . o Specimen source and specific site as appropriate for non-blood specimens (Microbiology, Histology, etc.) 4.1 Non-Cerner & Cerner Specimens delivered to the Central processing and specimen receiving laboratory are rejected for the following reasons: 4.1.1 The specimen received without patient identification label or unlabeled. Supplies. If all are negative 24 hours later, obtain 3 more. In excess of 200,000 specimens are processed in the Microbiology Laboratory on an annual basis. Supervisory Staff UW Medical Center - Montlake Microbiology Lab 206.598.6147. Objective : As a Microbiology Lab Assistant, responsible for assisting technical staff in the areas of chemistry, hematology, coagulation, urinalysis, specimen processing, or other areas where required. Section 1. In HD Isenberg (ed) Clinical Microbiology Procedures Handbook. Pre-processing tissue specimens with a tissue homogenizer: clinical and microbiological evaluation Erlangga Yusuf*, Marieke Pronk and Mireille van Westreenen Abstract Background: Tissues are valuable specimens in diagnostic microbiology because they are often obtained by invasive methods, and effort should thus be taken to maximize . Aerobic bacteriology, 1.2.1-4. Processing of these specimens showed PCR inhibition, possibly due to the presence of sodium polyanetholesulfonate which is a known inhibitor of PCR. › Facilitates automation and elutes samples in seconds. The microbiology director will evaluate the fellow for accuracy and completion of Microbiology specimen unknowns. Standard recommendations for most microbiology specimens are that if the specimen cannot be processed within one to two hours of collection, they must be refrigerated. The specimen receiv-ing and processing department is the largest Processing: ( surface area of specimen to expose the micro-organism) placed in sterile Petri dish add few drops of sterile water by sterile scalpel cut the sample into 1 mm pieces inoculate on surface of culture media as IMA & SABHI Avoid using tissue grinder (destruction of hyphal form especially zygomycetes) 6. •Use sterile technique in collecting the specimen. Stool: a. Non-fasting specimens often contain fat particles that can interfere with many analytical procedures. The clinical microbiology laboratory is responsible for providing guidance on how to select, collect, label, and transport specimens for processing in the laboratory. Place in a sterile container. PROCESSING OF URINE. 4. VI. Bacterial meningitis is the result of an infection of the meninges. Read Online 5.9 MB Download. Specimen collection • Proper specimen collection is one of the most important factors in diagnosing infection • Follow instructions for collecting and transporting specimens for microbiology tests • The best specimens are tissue, aspirate, pus, body fluid • Swabs are low yield and prone to contamination E-swab WASP ® — the Walk Away Specimen Processor — is the only automated instrument that addresses most aspects of microbiology specimen management. 3. specimen. Johns Hopkins Medical Microbiology Specimen Collection Guidelines - Updated 6/2019 5 6. Specimens from sites containing normal microbiota must be handled . Download Microbiology Requisition (.pdf) » . A Guide to Specimen Management in Clinical Microbiology is the classic reference that addresses and meets the needs of everyone in the total testing process circle. As far as possible, collect specimens before antimicrobial therapy and/or before application of antiseptic dressing. 3375 Hillview Avenue Palo Alto, CA 94304. Refer to Table 8, Set-up of microbiology specimens in MICROBIOLOGY PROCEDURE: MANAGEMENT OF MICROBIOLOGY SPECIMENS [Para-Link] for the list of processing equipment, culture media, and processing techniques used in the processing of stool and rectal swabs for enteropathogens. •Wait 48 hours after cessation of antimicrobial agents to obtain culture specimens. Specimens to Spec Proc Dept Specimens Picked Upat Client or PSC Specimen Collection SIPOC & Project Scope Specimens Removed from bags and Logged into LIS . Nutrient, ferric citrate; Esculin hydrolysis by group D strep turns media brown; sodium desoxycholate inhibits many bacteria. Test results from all laboratory disciplines can be affected by hemolysis, especially chemistry. specimen types (sputum, blood, urine, stool). › Only liquid-based multipurpose system that maintains viability of aerobic, anaerobic, and fastidious bacteria. USPS and Courier. First-morning specimen should be discarded. A The old adage of garbage in-garbage out applies here.High-quality specimens result in high-quality lab results.Even though the laboratory professional does not usually perform the preanalytic process of collecting microbiology specimens,it directly affects the outcome. - Processing - Clinical - AP - Microbiology - Medical Other Resources - Six Sigma BBs & GBs - Facilities Manager - Safety Officer - Medical Director - IT Manager/Director - HR Director Storage should not be in a frost free refrigerator. TML/MSH Department of Microbiology Laboratory P olicy & Procedure Manual Policy QPCMI06003.02 Page 2 of 40 Section: Microbiology Lab Specimen Management Manual Subject Title: Specimen Processing Procedure TORONTO MEDICAL LABORATORIES/MOUNT SINAI HOSPITAL, DEPARTMENT OF MICR OBIOLOGY Quality of the specimen • Inappropriate specimens: - Submission of contaminated specimens - Delay in specimen delivery - Viral culture without transport media - Collection of specimens from inappropriate body sites • =>Collection of microbiology specimens is generally not under direct control of lab. Question. tion of the microbiology laboratory. High standardization and traceability Copan WASP: Walk-Away Specimen Processor® › Automates all core aspects of microbiology specimen processing. It is important that specimen types (sputum, blood, urine, stool). Change if torn/damaged. Length: (Part time) Contract term is 6 months, with high possibility of extension Location: Kitchener, ON Job Summary: Prepare a variety of microbiological culture media, reagents and stains. Methods for the laboratory detection of other enteric pathogens can be found in other manuals, such as the American Society for Clinical Microbiology's Manual of Clinical Microbiologyor the World Health Organization's Manual for the UNDER REVIEW A Guide to Specimen Management in Clinical Microbiology is the classic reference that addresses and meets the needs of everyone in the "total testing process" circle. Specimens will be stored To process clinical specimens satisfactorily for bacteriological culture, consideration must be given to. 2 sample specimen timetable 6 3 specimen flowchart 7 4 laboratory biosafety and infection control 8 5 specimen collection, storage, and transport 11 6 receipt and login of sputum specimens in the laboratory 15 7 processing sputum for smear microscopy and qualitative culture 19 8 acid-fast bacilli microscopy (afb) preparation and staining 25 SPECIMEN PROCESSING If only blood culture bottles were received, subculture immediately to CHOC If little specimen is received, inoculate only CHOC and omit Gram stain For peritoneal fluid contaminated with bowel content add other selective media and omit broth cultures fecal specimens:Shigella, Vibrio cholerae O1/O139, and Salmonella serotype Ty phi. They are more reliably isolated if the clinical specimens are examined as soon as possible after collection . •Be aware of hand motions and what you touch To urine specimen, add an equal part of 50% to 70% alcohol. Specimen Processing staff also ship patient samples to outside reference laboratories if the procedure is not performed in one of our laboratories. Place a drop of the specimen onto each medium e. RCM, blood agar and MAC. Bile Esculin Agar (BEA) purpose. Specimen Specimen Type: Peritoneal dialysis fluid Container: Sterile container Volume: 10 -50 mL fluid Collection: Aseptically collect 10 -50 mL of peritoneal dialysis fluid. Customer Service Phone: 1-877-717-3733 . Ideally, a minimum volume of 1mL (up to 5 mL) of pus should be collected. INTRODUCTION. A fasting specimen is preferred for the majority of tests . Frequent, preventable medical errors can have an adverse effect on patient safety and quality as well as leading to wasted resources. A microcomputer system for use in the sequential processing of specimens has been developed in a hospital microbiology department which receives 90 000 specimens a year. 2. Processing of urine in microbiology by Rahul raj. That is why it's imperative to understand the basics of specimens and processing. d. Microbiology will check quality of specimen before plating bacterial culture and request new one if found inadequate. Today's topic in microbiology we will talk about specimen collection and agar plates (media). WASP ® DT is designed to mimic a technologist, utilizing industry best practices for planting and streaking patient specimens.. LIS driven sample processing protocols automatically select appropriate loop size (1µl, 10µl or 30µl loops) If a specimen is to be collected through intact skin, cleanse the skin first. Ramsamy Y , Mlisana KP. This preanalytical phase of testing is critical… 302 General Principles of Specimen Collection and Transport . Out of a total 103 culture specimen received for analysis in the NGS laboratory, 6 specimens were excluded from the final analysis. It provides complete, concise information on the unique needs of the microbiology laboratory regarding specimen management and is the only single source for the specimen management policies required for laboratory results that are . Freezing at -20°C may be used for many specimens if the period of storage is brief. SPECIMEN PROCESSING If only blood culture bottles were received, subculture immediately to CHOC If little specimen is received, inoculate only CHOC and omit Gram stain For peritoneal fluid contaminated with bowel content add other selective media and omit broth cultures c. Subacute endocarditis. The reports are dependent on the quality of the specimen and the time between the collection and. › Only liquid-based multipurpose system that maintains viability of aerobic, anaerobic, and fastidious bacteria. NABH STANDARDS FOR HOSPITALS • The programme is made to fulfill major objectives of the AAC.7 Clause C on Policies and procedures guide collection, identification, handling safe transportation, processing and disposal of . Additionally the specimen should ideally reach the laboratory within 24 hours of collection and be kept cold during transport. Specimen Collection and Preparation Guide 10/14/2019 mayocliniclabs.com Page 4 of 19 Blood Specimens Step 1: Determine the Specimen Requirements In the Test Catalog, use the Specimen and Overview tabs of each test to identify: • Patient preparation requirements The specimen receiving and processing department is the key entry point at the Riyadh Regional Laboratory (RRL) of the Ministry of Health. Inadequate amounts of specimen may yield false-negative results. Bile Esculin Agar (BEA) components. RRL consists of around 14 departments. 5 of these specimens were blood culture specimens. Specimen containers and requisitions will be delivered to the Clinical Microbiology Central Processing Area (CPA) within the specified period (depending on the specimen source and type) of collection. Consider gloves dirty. The concept of trans- Specimen collection devices supplied by DLO are to be used . Rationale Studies show that up to 56% of laboratory errors occur during the pre-analytical phase of testing1. Obtain 3 blood cultures on day 1 (15 minutes or more apart). Telephone reporting of laboratory results is provided on a limited basis and should be requested only when access to a patient's chart or to a computer terminal is not feasible. MICROBIOLOGY TESTS AND SPECIMEN COLLECTION GUIDELINES GENERAL CULTURE GUIDELINES •Whenever possible, specimens should be obtained before antimicrobial agents have been administered. 3. Identify specimens that require digestion/decontamination in processing for mycobacterial culture. Data handling is performed directly through the computers without the use of cards or worksheets. To learn the normal flora of the body and to appreciate the problem of recog-nizing clinically significant organisms in a specimen con-taining mixed flora, students collect and culture their own specimens. 176 (86.6%) of the 203 clinical microbiology laboratories in Pennsylvania. Evaluation Process Goals and Objectives will be reviewed with the fellow at the beginning of each Copan WASP: Walk-Away Specimen Processor® › Automates all core aspects of microbiology specimen processing. Study guide in Microbiology for the different media, tests and specimen processing. And transport General Principles of specimen collection and transport equal part of 50 % to 70 %.! E. RCM, blood agar and MAC Cary-Blair media infective agents is of. Of commonly used microbiological specimen processing in microbiology pdf, identify the specimen should ideally reach the laboratory, errors occur. 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